We'd like to understand how you use our websites in order to improve them. Register your interest. These products can be analyzed by polyacrylamide gel electrophoresis and silver staining. DAF is rapid and sensitive and is independent of cloning and prior genetic characterization. Here we describe this new methodology, its application to plant genotyping, and its perspectives in DNA fingerprinting and genome mapping. This is a preview of subscription content, log in to check access.
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The use of DNA amplification fingerprinting DAF as a tool for monitoring mixed microbial populations in bioreactors was evaluated. Short 8-mer or mer oligonucleotides were used to prime DNA extracts from various biological reactors during polymerase chain reaction PCR amplification.
The reactors examined in this study included two sets of anaerobic stirred tank continuous flow bioreactors. One set of anaerobic reactors was operated under methanogenic conditions and one set was operated under sulfate-reducing conditions. The anaerobic reactor communities in the methanol-fed reactors showed extensive DAF homology.
DAF was also applied to a fixed-film azo dye degrading reactor to examine the degree of uniformity of colonization of the substratum in representative regions of the reactor. This method is a quick and relatively inexpensive means of monitoring microbial community structure during biological processes.
Since no cultivation of the sample is involved, the genetic profile of the community is not biased by outgrowth conditions. DAF profiles may be useful for comparisons of population changes over time or of bench-scale vs pilot-scale reactors but not adequate for assessing community diversity.
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Cite Favorites. Abstract The use of DNA amplification fingerprinting DAF as a tool for monitoring mixed microbial populations in bioreactors was evaluated. Similar articles Upflow anaerobic sludge blanket reactor--a review. Bal AS, et al. Indian J Environ Health. PMID: Review.
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DNA Amplification Fingerprinting Using Very Short Arbitrary Oligonucleotide Primers
U se of the polymerase chain reaction pcr , amplification of targeted nucleotide sequences as a means of identifying microorganisms for diagnostic and epidemiologic purposes 1 , 2 , has initiated a transformation in laboratory based approaches to infectious disease diagnosis and was the subject of an earlier LCDC report 3. The high sensitivity and specificity of properly targeted long chain primer pairs and the great variety of specimens amenable to PCR analysis, including nonculturable specimens such as paraffin embedded tissues, has generated numerous diagnostic applications 4. Another genetic approach has recently been suggested by two groups 5 , 6 in which PCR amplification of DNA polymorphisms was directed by single small primers only eight to 10 nucleotides in length. Following polyacrylamide gel electrophoresis page , these demonstrate an array of amplicons that yield a banding fingerprint that is characteristic for each combination of short primer and DNA source. This technique has been designated DNA amplification fingerprinting daf. Particularly important are the specialized silver staining method 8 required for resolution of small, as opposed to large, amplicons, and the primer concentrations required for effective priming. There appears to be some particular advantages to daf compared to the more traditional PCR approach.
DNA amplification fingerprinting: A strategy for genome analysis
Balyan, P. Sharma, B. Ramesh, Alok Kumar, Joy K. Roy, Rajeev K. Varshney and P. Ten arbitrary, 8- mer, GC rich, linear primers and ten 1 1-mer minihairpin primers, each having a 3-mer core sequence at 3' end, were utilized for DNA amplification. Nine linear primers and four mini-hairpin primers produced characteristic fingerprinting patterns involving polymorphism.